*Sensitive to detect low abundance proteins
*Prolonged shelf life up to 12 months
ECL solution generates a long-duration chemiluminescent signal from femto to pico level proteins, making possible extended exposure time and multiple exposures to film of low-abundance proteins. This ECL solution is stable for up to 12 months at 2-8 ºC.
Recommended antibody dilution: primary (1mg/ml stock) 1:2,000-20,000; secondary (1mg/ml stock solution) 1:5,000-50,000
1.25mM luminol, 2.0mM 4-iodophenylboronic acid, 5.3 mM propietary stabilized hydrogen peroxide in 100mM Tris/HCl, pH8.8
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